3E78
Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mh-p37
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.445, 68.761, 60.024 |
| Unit cell angles | 90.00, 108.04, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.186 |
| R-free | 0.24000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.022 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 29894 | |
| <I/σ(I)> | 16.3 | 5 |
| Completeness [%] | 96.2 | 93.2 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 3 | 298 | 0.1 M Ammonium Bromide, O.1 M Citric Acid, 40% PEG 4000, 9% N-decyl-beta-D-maltoside detergent , pH 3.0, Batch, temperature 298K |
