3E6J
Crystal Structure of Variable Lymphocyte Receptor (VLR) RBC36 in Complex with H-trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.221, 48.564, 55.477 |
| Unit cell angles | 90.00, 116.51, 90.00 |
Refinement procedure
| Resolution | 49.630 - 1.670 |
| R-factor | 0.14825 |
| Rwork | 0.146 |
| R-free | 0.18221 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r9u |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.620 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (- auto MR) |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.089 | 0.228 |
| Number of reflections | 28666 | |
| <I/σ(I)> | 13.8 | 3.7 |
| Completeness [%] | 95.3 | 71.1 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 1.9 M (NH4)2SO4, 0.1 M Sodium Citrate (pH 5.6), 0.2 M K/Na Tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
