3E5Y
Crystal structure of TrmH family RNA methyltransferase from Burkholderia pseudomallei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-02 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.724, 80.532, 80.882 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j85 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.724 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.4.0067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.140 | 0.652 |
Number of reflections | 12194 | |
<I/σ(I)> | 12 | 3 |
Completeness [%] | 97.9 | 95.9 |
Redundancy | 6.4 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 289 | PACT sparse matrix screen G10 and H10; 20% PEG 3350, 0.2 M sodium/potassium phosphate, 0.1 M BisTris propane, pH 7.5 or 8.5; 27.6 mg/mL protein; Crystal ID 109613h10, VAPOR DIFFUSION, SITTING DROP, temperature 289K |