3E5S
Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-31 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.172, 78.514, 60.949 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.167 |
| Rwork | 0.164 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3BDC.PDB |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.477 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.950 | 1.960 |
| High resolution limit [Å] | 1.859 | 1.859 |
| Rmerge | 0.066 | 0.322 |
| Number of reflections | 24052 | |
| <I/σ(I)> | 15.7 | 2.48 |
| Completeness [%] | 97.6 | 91.1 |
| Redundancy | 4.92 | 2.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 35% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
