3E50
Crystal structure of human insulin degrading enzyme in complex with transforming growth factor-alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0003 |
| Spacegroup name | P 65 |
| Unit cell lengths | 262.172, 262.172, 90.522 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.19274 |
| Rwork | 0.191 |
| R-free | 0.23133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.982 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.094 |
| Number of reflections | 157230 |
| <I/σ(I)> | 23.6 |
| Completeness [%] | 100.0 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
