3E46
Crystal structure of ubiquitin-conjugating enzyme E2-25kDa (Huntington interacting protein 2) M172A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97000 |
| Spacegroup name | I 4 |
| Unit cell lengths | 134.490, 134.490, 38.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.380 - 1.860 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bep |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.514 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 27414 | |
| <I/σ(I)> | 1.98 | |
| Completeness [%] | 93.4 | 52.4 |
| Redundancy | 4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | Calcium acetate, Sodium acetate, NaCl, PEG 8000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
