3E3V
Crystal structure of RecX from Lactobacillus salivarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.910, 42.712, 24.185 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 33.720 - 2.040 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| Starting model (for MR) | None |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 10853 | |
| <I/σ(I)> | 11.3 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.9 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 2M Ammonium sulfate, 0.1M Na-citrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
