3E3D
Structure of hen egg white lysozyme with the magic triangle I3C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.830, 76.830, 38.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.400 - 1.550 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.023 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | SHELXD |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.069 | 0.150 |
| Number of reflections | 17445 | |
| <I/σ(I)> | 38.2 | 21.2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 26.1 | 24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES, 15%(wt/wt) PEG 3350, 8mM sodium I3C(magic triangle) solution, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
