3E2B
Crystal structure of Dynein Light chain LC8 in complex with a peptide derived from Swallow
Replaces: 2P1KExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 77 |
Detector technology | AREA DETECTOR |
Collection date | 2008-02-27 |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 44.158, 44.158, 203.718 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.810 - 2.000 |
R-factor | 0.22084 |
Rwork | 0.216 |
R-free | 0.26599 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2p1k |
RMSD bond length | 0.007 |
RMSD bond angle | 0.880 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.700 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 10184 | |
<I/σ(I)> | 23.5 | 5.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.7 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2M sodium potassium tartrate, 0.1M sodium citrate, 2.0M ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |