3E1K
Crystal structure of Kluyveromyces lactis Gal80p in complex with the acidic activation domain of Gal4p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-06 |
| Detector | SBC-3 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.100, 160.500, 132.600 |
| Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nvw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.098 | 0.249 |
| Number of reflections | 75005 | |
| <I/σ(I)> | 7.2 | 2 |
| Completeness [%] | 88.5 | 55.8 |
| Redundancy | 2.4 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES, 20-25% (w/v) pentaerythritol propoxylate 5/4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
