3E17
Crystal structure of the second PDZ domain from human Zona Occludens-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.177, 41.325, 41.292 |
| Unit cell angles | 80.05, 68.63, 68.52 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fne |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.498 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 15928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 283 | 12% PEG8000, 0.1M Tris-HCl, pH8.3, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
