3E13
Iron reconstituted ferric binding protein from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-3 |
Synchrotron site | SSRL |
Beamline | BL11-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.974609 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.113, 54.740, 92.237 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.800 - 1.600 |
R-factor | 0.14677 |
Rwork | 0.145 |
R-free | 0.18595 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y4t |
RMSD bond length | 0.018 |
RMSD bond angle | 1.581 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.106 | 0.196 |
Number of reflections | 38962 | |
<I/σ(I)> | 8.6 | 3 |
Completeness [%] | 90.9 | 73.9 |
Redundancy | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 24% PEG 4000, 0.1M Tris-HCl, 60 mM sodium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |