3E0O
Crystal structure of MsrB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97182 |
Spacegroup name | P 3 |
Unit cell lengths | 136.114, 136.114, 61.925 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.790 - 2.600 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.29400 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.800 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.101 | 0.436 |
Number of reflections | 39083 | |
<I/σ(I)> | 12.5 | 2.8 |
Completeness [%] | 99.4 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 298 | 1.2M Lithium sulfate, 0.1M HEPES, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 298 | 1.2M Lithium sulfate, 0.1M HEPES, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |