3E0M

Crystal structure of fusion protein of MsrA and MsrB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97950, 0.97962, 0.97182
Spacegroup name	P 21 21 2
Unit cell lengths	158.521, 165.489, 77.281
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.400
R-factor	0.239
Rwork	0.239
R-free	0.28200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.057	0.279
Number of reflections	77162
<I/σ(I)>	37.9	2.6
Completeness [%]	91.2	49.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	0.1M MES (pH 5.6), 1% (w/v) PEG 4000, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	0.1M MES (pH 5.6), 1% (w/v) PEG 4000, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K