3E04
Crystal structure of human fumarate hydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 188.483, 188.483, 114.632 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.19728 |
| Rwork | 0.197 |
| R-free | 0.24408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yfm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.419 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.140 | |
| Number of reflections | 168629 | |
| <I/σ(I)> | 9.7 | 2 |
| Completeness [%] | 99.3 | 96.8 |
| Redundancy | 6.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20% PEG 3350, 10% ethanediol, 0.2 M sodium acetate, 0.1 M bis-tris propane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
