3DZP
Thaumatin by LB nanotemplate method after high X-Ray dose on ESRF ID29 beamline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.796, 57.796, 150.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.790 - 1.510 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rqw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.105 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.936 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 | 0.502 |
| Total number of observations | 45476 | |
| Number of reflections | 41813 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM ADA, 1M Na/K-tartrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
