3DZE
Crystal structure of bovine coupling Factor B bound with cadmium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.010, 49.924, 36.942 |
| Unit cell angles | 90.00, 109.58, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.150 |
| R-factor | 0.1305 |
| Rwork | 0.129 |
| R-free | 0.15496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.322 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.036 | 0.304 |
| Number of reflections | 62817 | |
| <I/σ(I)> | 32.2 | 1.84 |
| Completeness [%] | 83.5 | 11.3 |
| Redundancy | 3.3 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 50% PPG 400, 100 mM Tris, pH 7.4-8.0, vapor diffusion, temperature 277K |
