3DYL

human phosphdiesterase 9 substrate complex (ES complex)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	103.787, 103.787, 269.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.670 - 2.700
R-factor	0.19089
Rwork	0.188
R-free	0.21902
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.023
RMSD bond angle	1.931
Refinement software	REFMAC (5.3.0008)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.670	2.800
High resolution limit [Å]	2.700	2.700
Rmerge		0.771
Number of reflections	37247
<I/σ(I)>		2
Completeness [%]		100
Redundancy		7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1