3DXJ
Crystal structure of thermus thermophilus rna polymerase holoenzyme in complex with the antibiotic myxopyronin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 |
| Unit cell lengths | 235.090, 235.090, 250.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.28900 |
| Structure solution method | MR |
| Starting model (for MR) | 1iw7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.570 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.700 |
| Number of reflections | 150814 | |
| <I/σ(I)> | 10.8 | 1.33 |
| Completeness [%] | 96.0 | 77.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.85 | 298 | MES, MAGNESIUM FORMATE, PEG 8000, SPERMINE, pH 5.85, VAPOR DIFFUSION, temperature 298K |
