3DX9
Crystal Structure of the DM1 TCR at 2.75A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.740, 70.727, 154.412 |
| Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
| Resolution | 154.300 - 2.750 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.490 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 154.300 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 22674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Hepes, Magnesium Formate, PEG 3350, pH 7.0, vapor diffusion, hanging drop, temperature 293K |
