3DWJ
Heme-proximal W188H mutant of inducible nitric oxide synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-10-31 |
| Detector | NOIR-1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 214.597, 214.597, 111.933 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.770 - 2.750 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nod |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.486 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (phenix) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.108 | 0.423 |
| Number of reflections | 37844 | |
| <I/σ(I)> | 6.7 | 2.1 |
| Completeness [%] | 95.0 | 94.4 |
| Redundancy | 4.53 | 4.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 277 | 0.7-1.2M (NH4)2SO4, 0.1M MES, pH 4.9-5.7, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
