3DWD
Crystal structure of the ArfGAP domain of human ARFGAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 6 |
| Unit cell lengths | 82.422, 82.422, 81.008 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gf9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.445 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.092 | 0.037 | 0.653 |
| Number of reflections | 12298 | ||
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 99.6 | 97.4 | 98.3 |
| Redundancy | 7.4 | 7.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 3.5M Sodium Formate, 0.1M Tris, 1:100 chymotrypsin, pH 8.5, vapor diffusion, sitting drop, temperature 291K |
