3DVU
Crystal structure of the complex of murine gamma-herpesvirus 68 Bcl-2 homolog M11 and the Beclin 1 BH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-16 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97874 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.424, 53.134, 64.059 |
| Unit cell angles | 90.00, 96.67, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ABO with residues 52-73 removed. |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.156 | 0.420 |
| Number of reflections | 22424 | |
| <I/σ(I)> | 6.8 | 1.6 |
| Redundancy | 2.7 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 20% PEG 3350, 0.1M sodium acetate, pH 4.5, 10mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
