3DVO
SgrAI with cognate DNA and calcium bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.136, 119.248, 123.799 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 41.068 - 1.892 |
| R-factor | 0.2211 |
| Rwork | 0.220 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dpg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.255 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.890 |
| Rmerge | 0.120 |
| Number of reflections | 126080 |
| <I/σ(I)> | 6.5 |
| Completeness [%] | 92.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | PEG4000, buffer, NaCl, CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 2 | PEG4000 | ||
| 5 | 1 | 2 | NaCl | ||
| 6 | 1 | 2 | CaCl2 |
