3DVG
Crystal structure of K63-specific fab Apu.3A8 bound to K63-linked di-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2008-01-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97607 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.806, 88.117, 90.226 |
| Unit cell angles | 90.00, 108.28, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.600 |
| R-factor | 0.22355 |
| Rwork | 0.219 |
| R-free | 0.26079 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 24012 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 98.1 | 97.9 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291 | protein: 17.0 mg/mL in 20 mM Tris-HCl pH 7.3, 150 mM NaCl well solution: 0.1M Tris-HCl pH 8.0, 1.6M LiS04 , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
