3DU6

Structure of the catalytic subunit of telomerase, TERT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	298
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	85.042, 122.657, 212.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.710
R-factor	0.24038
Rwork	0.238
R-free	0.27763
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	0.848
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.710
Number of reflections	56173
<I/σ(I)>	9.3
Completeness [%]	97.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1