3DU3
E(L212)A, D(L213)A, A(M249)Y triple mutant structure of photosynthetic reaction center
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2001-09-13 |
Detector | CUSTOM-MADE |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 141.100, 141.100, 187.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
Rwork | 0.191 |
R-free | 0.20140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6n |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.102 | 0.278 |
Number of reflections | 49481 | |
<I/σ(I)> | 15.3 | 3.4 |
Completeness [%] | 95.0 | 89 |
Redundancy | 2.6 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Potassium phosphate, LDAO, Heptane triol, Dioxane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |