3DTK
Crystal structure of the IRRE protein, a central regulator of DNA damage repair in deinococcaceae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.260, 52.672, 63.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 3.240 |
| R-factor | 0.22853 |
| Rwork | 0.226 |
| R-free | 0.27702 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dte |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.867 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.420 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Number of reflections | 4827 | |
| <I/σ(I)> | 13.1 | 2.2 |
| Completeness [%] | 97.7 | 97.9 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 281 | 20% PEG 3350, 0.2M Potassium fluoride, pH 7.5, VAPOR DIFFUSION, temperature 281K |
