3DT4
The structure of rat cytosolic PEPCK in complex with oxalate and GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.454, 119.759, 87.300 |
| Unit cell angles | 90.00, 96.70, 90.00 |
Refinement procedure
| Resolution | 30.020 - 1.450 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.20300 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.601 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 3.120 | 1.450 |
| Rmerge | 0.080 | 0.045 | 0.717 |
| Number of reflections | 217132 | ||
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 99.8 | 99.6 | 98 |
| Redundancy | 7.1 | 7.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor diffusion | 7.4 | 298 | 12-30% PEG 3350, 0.1M HEPES, 10 MM MNCL2, pH 7.4, hanging drop vapor diffusion, temperature 298K |
