3DSZ
Engineered human lipocalin 2 in complex with Y-DTPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 82.350, 82.350, 115.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| Rwork | 0.213 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.979 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.109 | 0.372 |
| Number of reflections | 26827 | |
| <I/σ(I)> | 4.1 | 6.1 |
| Completeness [%] | 98.2 | 97.4 |
| Redundancy | 9.7 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 22% polyethylene glycol 3350, 0.1M Bistris/HCl, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
