3DSX

Crystal structure of RabGGTase(DELTA LRR; DELTA IG)in complex with di-prenylated peptide Ser-Cys(GG)-Ser-Cys(GG) derivated from Rab7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9841
Spacegroup name	P 21 21 21
Unit cell lengths	67.354, 91.153, 114.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.370 - 2.100
R-factor	0.17056
Rwork	0.168
R-free	0.22942
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DB ENTRY 3DSS
RMSD bond length	0.011
RMSD bond angle	1.285
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	41826
<I/σ(I)>	23.4	6.9
Completeness [%]	99.8	100
Redundancy	7.2	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	284	14% (w/v) PEG 3350, 0.2M Ca(OAc)2, 2.5% (v/v)DMSO, 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 284K