3DSW
Crystal structure of RabGGTase(DELTA LRR; DELTA IG)in complex with mono-prenylated peptide Ser-Cys(GG)-Ser-Cys derivated from Rab7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9825 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.051, 90.908, 114.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.210 - 2.150 |
| R-factor | 0.17278 |
| Rwork | 0.170 |
| R-free | 0.23154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dss |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.446 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 38414 | |
| <I/σ(I)> | 15.2 | 5.2 |
| Completeness [%] | 99.1 | 99.8 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 284 | 14% (w/v) PEG 3350, 0.2M Ca(OAc)2, 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 284K |
