3DSO
Crystal structure of Cu(I) bound copper resistance protein CopK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 32.193, 84.672, 23.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.800 - 1.550 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.582 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.043 | 0.042 | 0.129 |
| Rmeas | 0.043 | ||
| Total number of observations | 87158 | ||
| Number of reflections | 9911 | ||
| <I/σ(I)> | 27.1 | 13.5 | |
| Completeness [%] | 99.1 | 98.2 | 93.7 |
| Redundancy | 8.8 | 8.8 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M KSCN, 32% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
