3DSG
XC1028 from Xanthomonas campestris Adopts a PilZ Domain-like Structure Yet with Trivial c-di-GMP Binding Activity
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920, 0.96398, 0.97888 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.896, 50.962, 92.745 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 25.480 - 2.090 |
| R-factor | 0.277 |
| Rwork | 0.277 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.040 |
| RMSD bond angle | 4.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.100 |
| Rmerge | 0.044 | 0.220 |
| Number of reflections | 22930 | |
| <I/σ(I)> | 27.3 | 5.3 |
| Completeness [%] | 94.7 | 79 |
| Redundancy | 5.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 297 | 0.1M sodium cacodylate pH 6.5, 18% PEG 2KMME, 2% glycerol, VAPOR DIFFUSION, temperature 297K |
