3DS7
Structure of an RNA-2'-deoxyguanosine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.139, 41.830, 64.810 |
| Unit cell angles | 86.55, 81.16, 89.64 |
Refinement procedure
| Resolution | 19.500 - 1.850 |
| R-factor | 0.205 |
| Rwork | 0.200 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1U8D |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.000 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.075 | 0.297 |
| Number of reflections | 29177 | |
| <I/σ(I)> | 8.4 | 3.4 |
| Completeness [%] | 94.0 | 91.4 |
| Redundancy | 3.56 | 3.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.01M K-HEPES, 0.0119 M cobalt(III) hexammine, 22% PEG 2000, 0.66M ammonium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | K-HEPES | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | cobalt(III) hexammine | ||
| 3 | 1 | 1 | PEG 2000 | ||
| 4 | 1 | 1 | ammonium acetate | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | K-HEPES | ||
| 7 | 1 | 2 | cobalt(III) hexammine | ||
| 8 | 1 | 2 | PEG 2000 | ||
| 9 | 1 | 2 | ammonium acetate |
