3DR5
Crystal structure of the Q8NRD3_CORGL protein from Corynebacterium glutamicum. Northeast Structural Genomics Consortium target CgR117.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 50.511, 50.511, 303.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.300 - 2.250 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.087 | 0.283 |
| Number of reflections | 19601 | |
| <I/σ(I)> | 22.3 | 5.6 |
| Completeness [%] | 93.4 | 69.2 |
| Redundancy | 20.1 | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 291 | 10% PEG 4000, 0.1M magnesium chloride, 0.1M MES, pH6.15, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
