3DQQ
The crystal structure of the putative tRNA synthase from Salmonella typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 254.541, 254.541, 94.459 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 127.000 - 2.700 |
| R-factor | 0.18948 |
| Rwork | 0.188 |
| R-free | 0.21048 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.718 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 127.000 | 2.771 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.158 | 0.760 |
| Number of reflections | 47157 | |
| <I/σ(I)> | 25.71 | 2.5 |
| Completeness [%] | 1.0 | 0.9956 |
| Redundancy | 25.1 | 22.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Bis/Tris, 3M NaCl, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
