3DPR

Human rhinovirus 2 bound to a concatamer of the VLDL receptor module V3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	498.117, 498.117, 658.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.500
R-factor	0.426
Rwork	0.425
R-free	0.44100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.199	0.462
Number of reflections	777511
<I/σ(I)>	5
Completeness [%]	48.3	11.8
Redundancy	2.4	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	Ammonium sulphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K