3DPG
SgrAI with noncognate DNA bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.726, 78.621, 86.834 |
| Unit cell angles | 90.00, 109.76, 90.00 |
Refinement procedure
| Resolution | 31.257 - 1.910 |
| R-factor | 0.1867 |
| Rwork | 0.185 |
| R-free | 0.22540 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.958 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | DM |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.910 |
| Rmerge | 0.071 |
| Number of reflections | 66598 |
| <I/σ(I)> | 6.8 |
| Completeness [%] | 96.1 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | PEG4000, NaCl, CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 2 | PEG4000 | ||
| 5 | 1 | 2 | NaCl | ||
| 6 | 1 | 2 | CaCl2 |
