3DOM
Crystal Structure of the complex between Tfb5 and the C-terminal domain of Tfb2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.583, 103.593, 114.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.700 - 2.600 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.26100 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.826 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.058 | 0.041 | 0.131 |
| Number of reflections | 13927 | ||
| <I/σ(I)> | 23.7 | ||
| Completeness [%] | 96.4 | 89.7 | 91.9 |
| Redundancy | 4 | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG3350 MME, NaCl, Hepes, pH 7.5, vapor diffusion, sitting drop, temperature 293K |
