3DOJ
Structure of Glyoxylate reductase 1 from Arabidopsis (AtGLYR1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 156.543, 156.543, 75.467 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.610 - 2.101 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uyy |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.817 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.410 |
| Number of reflections | 27496 | |
| <I/σ(I)> | 11.5 | 4.1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8.4 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M calcium acetate hydrate, 20% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
