3DNJ

The structure of the Caulobacter crescentus ClpS protease adaptor protein in complex with a N-end rule peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97927
Spacegroup name	P 1 21 1
Unit cell lengths	33.693, 53.974, 44.824
Unit cell angles	90.00, 110.39, 90.00

Refinement procedure

Resolution	14.876 - 1.150
R-factor	0.136
Rwork	0.135
R-free	0.15900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1R60 chain C
RMSD bond length	0.006
RMSD bond angle	0.876
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.190
High resolution limit [Å]	1.150	2.480	1.150
Rmerge	0.078	0.061	0.194
Number of reflections	51120
<I/σ(I)>	8.1
Completeness [%]	95.6	95.2	69.5
Redundancy	6.3	6.4	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.1 M BIS-TRIS PH 5.5 0.2 M MGCL2 13% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K