3DNB
HELIX GEOMETRY, HYDRATION, AND G.A MISMATCH IN A B-DNA DECAMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET P1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 32.520, 26.170, 34.300 |
| Unit cell angles | 90.00, 118.90, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.300 |
| R-factor | 0.164 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.027 * |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 9 (mg/ml) | |
| 2 | 1 | drop | 0.7 (M) | ||
| 3 | 1 | reservoir | MPD | 45 (%(v/v)) |
