3DMU
Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-20 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.220, 48.220, 63.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.198 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EY8.PDB |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.080 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0017) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.980 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.030 | 0.284 |
| Number of reflections | 14710 | |
| <I/σ(I)> | 30.16 | 4.05 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 10.6 | 3.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 44% MPD, 25 mM Potassium Phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
