3DMF
T. Thermophilus 16S rRNA N2 G1207 methyltransferase (RsmC) in complex with AdoMet
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.697, 88.421, 95.417 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.580 |
R-factor | 0.1864 |
Rwork | 0.185 |
R-free | 0.22020 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.322 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.051 | 0.398 |
Number of reflections | 55718 | |
<I/σ(I)> | 27.3 | 3 |
Completeness [%] | 98.6 | 92.4 |
Redundancy | 5.9 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch technique under oil | 7.5 | 277 | 0.1 M HEPES (pH 7.5) 1.5 M lithium sulfate monohydrate, microbatch technique under oil, temperature 277K |