3DMD
Structures and Conformations in Solution of the Signal Recognition Particle Receptor from the archaeon Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2006-08-31 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.956, 102.368, 101.766 |
| Unit cell angles | 90.00, 119.81, 90.00 |
Refinement procedure
| Resolution | 65.060 - 2.210 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dm9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.939 |
| Data reduction software | MOSFLM (in ELVES) |
| Data scaling software | SCALA (in ELVES) |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.400 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.074 | 0.766 |
| Number of reflections | 65992 | |
| <I/σ(I)> | 8.9 | 1.4 |
| Completeness [%] | 98.8 | 97.1 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.9-1.2M Lithium Sulfate, 0.4-0.6M Ammonium Sulfate, 100 mM Na Citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
