3DLX
Crystal structure of human 3-oxoacid CoA transferase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.956, 168.492, 95.322 |
| Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
| Resolution | 48.110 - 2.200 |
| R-factor | 0.17887 |
| Rwork | 0.178 |
| R-free | 0.22473 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m3e |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.556 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.110 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 0.526 |
| Number of reflections | 92253 | |
| <I/σ(I)> | 7.4 | 1.9 |
| Completeness [%] | 98.6 | 97 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 25% PEG 3350, 0.2 M NaCl, 0.1 M Tris-HCl, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
