3DLH
Crystal structure of the guide-strand-containing Argonaute protein silencing complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.744, 114.629, 175.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| Rwork | 0.226 |
| R-free | 0.28400 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.634 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.089 | 0.447 |
| Number of reflections | 30932 | |
| <I/σ(I)> | 14 | 2 |
| Completeness [%] | 99.3 | 95.8 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 308 | 18% PEG8000, 200mM KCl, 80mM MgAc2, 50mM Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 308K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Mes | ||
| 2 | 1 | 2 | Mes | ||
| 3 | 1 | 2 | KCl | ||
| 4 | 1 | 2 | MgAc2 | ||
| 5 | 1 | 2 | PEG8000 |
