3DLB
Crystal structure of the guide-strand-containing Argonaute protein silencing complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97918, 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.846, 123.399, 84.910 |
| Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| Rwork | 0.254 |
| R-free | 0.29310 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.643 | |
| Number of reflections | 42559 | |
| <I/σ(I)> | 29.4 | 1.72 |
| Completeness [%] | 100 | |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 308 | 5% PEGMME550, 0.1M KCl, 10mM MgCl2, 50 mM Tris HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 308K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 2 | 1 | 1 | Tris HCl | ||
| 3 | 1 | 2 | MgCl2 | ||
| 4 | 1 | 2 | Tris HCl | ||
| 5 | 1 | 2 | PEGMME550 | ||
| 6 | 1 | 2 | KCl |
