3DKM

Crystal structure of the HECTD1 CPH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-06-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	33.710, 45.564, 51.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.100 - 1.600
R-factor	0.15695
Rwork	0.156
R-free	0.17788
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dk3
RMSD bond length	0.016
RMSD bond angle	1.620
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	10709
<I/σ(I)>	40.84	11.103
Completeness [%]	98.1	87.1
Redundancy	9.7	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	20 % PEG 5000, 0.1 M BIS-TRIS, PH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K